细粒棘球绦虫铜锌超氧化物歧化酶基因的表达与特征分析

doi:10.11843/j.issn.0366-6964.2016.02.018

Abstract

Abstract:

The aim of this study was to characterise the Cu/Zn superoxide dismutase of Echinococcus granulosus (Eg-SOD)，and analyze its role in the parasite antioxidant system.The recombinant Eg-SOD was expressed in E.coli，and the enzymatic activity was measured，and analyses including western blotting，ELISA and immunofluorescence localization were also performed.The recombinant Eg-SOD was expressed as soluble protein，and its activity was up to （464.55 ±19.99） U•mg^-1 measured by hydroxylamine method.As showed by the result of western blot，the recombinant Eg-SOD could be recognized by the serum of mice secondary experimentally infected with E.granulosus protoscolexes，and showed a high immunogenicity；ELISA tests showed that recombinant Eg-SOD could recognize all the sera from mice experimentally infected with protoscolexs and from sheep naturally infected with E.granulosus，and have 37.5% cross-reactions with sera from sheep infected with Cysticercus tenuicollis，which indicates Eg-SOD might be a potential diagnose antigen.The immunolocalization of Eg-SOD showed a wide extent of distribution，mostly in the tissue clearance of adult worms and protoscolexes，and fewer on the tegument.These results provide the fundamental understanding of Eg-SOD，and revealed its capable of antioxidation effect.

CLC Number:

S852.734

SONG Xing-ju，HU Dan-dan，ZHONG Xiu-qin，YANG Ai-guo，GUO Li，MAO Guang-qiong，WANG Ning，YAN Min，WANG Tao，GU Xiao-bin，YANG Guang-you. Prokaryotic Expression and Characterization of Cu/Zn Superoxide Dismutase of Echinococcus granulosus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 346-353.

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Prokaryotic Expression and Characterization of Cu/Zn Superoxide Dismutase of Echinococcus granulosus

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